Kit ɗin Haɓakar DNA na Ƙaƙwalwar Ƙaƙwalwar Ƙaƙwalwar Ƙwaƙwalwar Ƙwararren Ƙwararren Ƙwararriyar DNA Mai Sauƙi
Gabatarwar Samfur:
Kit ɗin cirewar DNA na Iclean yana ba da sauri da ingantaccen hanyar ƙwanƙwasa maganadisu don tsarkakewa & cire DNA (ciki har da genomic, mitochondrial da DNA viral) daga kyallen da aka adana, ɗigon ruwa, ruwan jiki, da buccal, mahaifa, ƙwayoyin fata, ƙwayoyin cuta da sauransu.
Za'a iya adana samfuran halitta a cikin keɓantaccen tanadin ajiyar mu har zuwa kwanaki 30 a zafin daki kafin aiki ba tare da hasarar gani ba a yawan amfanin DNA ko inganci (） kwanaki 30 idan an adana a cikin yanayin sanyi)
Ana iya tsarkake DNA mai inganci mai inganci a cikin mintuna 15 ba tare da cirewar phenol/chloroform ko hazo barasa ba, tare da matsakaicin yawan amfanin DNA na 8 μg a kowace buccal swab.DNA da aka tsarkake, tare da kusan 20-30 kb, ya dace da aikace-aikacen ƙasa kamar PCR ko wasu halayen enzymatic.
Amfanin Samfur
Babban inganci, takamaiman hakar DNA guda ɗaya, haɓakar kawar da furotin na ƙazanta da sauran mahaɗan kwayoyin halitta a cikin sel.Rubutun DNA da aka fitar suna da girma, babban tsabta, barga kuma abin dogara cikin inganci.
1. Fasaha na tushen Magnetic don ware DNA na genomic ba tare da buƙatar sinadarai masu haɗari, centrifugation, ko manifolds, phenol da hazo ethanol.
2.Rapid da ingantaccen tsarkakewa na genomic DNA daga buccal swabs na mutum a cikin ƙasa da mintuna 15 bayan shirye-shiryen samfurin da lysis.
3.Simple lysis tare da Proteinase K ba tare da buƙatar kowane lysis na inji ba.
4.Ƙarancin gurɓata da RNA.
5.The tsarkake genomic DNA nuna ingantattun ayyuka na ƙasa a cikin aikace-aikace ciki har da PCR.
6. Ya haɗa da kit ɗin da aka tsara don sarrafa kansa ta atomatik na samfurori masu yawa a cikin faranti 96-riji ta amfani da robot mai sarrafa ruwa.
Amfanin Samfur:
Don tsarkakewa & warewa na DNA (ciki har da genomic, mitochondrial, kwayan cuta, parasite & viral DNA) daga kyallen takarda, saliva, ruwan jiki, da buccal, mahaifa, ƙwayoyin fata, ƙwayoyin ƙwayoyin cuta, nama, swabs, CSF, ruwan jiki, ƙwayoyin fitsari da aka wanke. .
Umarnin Samfura
1. Kafin amfani da nucleic acid cire reagents
①.Canja wurin kaushi na proteinase k cikin busasshiyar foda mai ɗauke da proteinase k a gauraya sosai.
②.Ƙara 18ml da 42ml na cikakkar ethanol zuwa CY3 da CY4 na samfurin CY-F006-10 (50preps-cells) da CY-F006-20 (50preps-saliva), sai a gauraya da kyau.
③.Ƙara 36ml da 84ml cikakkar ethanol zuwa CY3 da CY4 na samfurin CY-F006-11 (100preps-cells) da CY-F006-21 (100preps-saliva) sai a gauraya da kyau.
2. Matakan hakar swab:
①Swab busassun tarin, ƙara 0.6ml CY1 ruwa, 10ul proteinase K, Mix da kyau, sanya shi a cikin iska incubator a 65 digiri Celsius da kuma shirya na 30 minutes (ko rigar tarin: samfurin centrifuge tube dauke da swab da adana bayani ne centrifuged a 12000 rpm na minti 1 , Ci gaba da hazo, cire supernatant, ƙara 0.6ml CY1 ruwa, 10ul proteinase k, gauraya sosai, sanya shi a cikin injin iska a 65 ° C kuma a sanya shi tsawon minti 30).
②.Cire swab da centrifuge a 12000rpm na minti 1.
③.Fitar da duk abin da ke sama zuwa sabon bututun centrifuge kuma yi gwaji.
④.Sai a zuba ruwa mai 0.25ml CY2, 10ul Magnetic beads* (an girgiza sosai kafin a yi amfani da shi) sai a gauraya sosai na tsawon mintuna 12, sai a daka shi a kan ma'aunin maganadisu sai a bar shi ya kai 30s, sai a tsotse ruwan.
⑤A zuba 0.6ml na ruwa CY3, a gauraya da kyau na tsawon mintuna 3, sai a dora a kan ma'aunin maganadisu sai a bar shi ya tsaya tsawon 30s don tsotse ruwan.
⑥.Sai ki zuba 0.6ml na ruwa CY4, ki gauraya na tsawon minti 3, sai ki dora shi a kan ma'aunin maganadisu sai a bar shi ya tsaya tsawon 30s, sai a tsotse ruwan.
⑦.Maimaita matakai ②⑥
⑧.A bushe don 10-20min a dakin da zafin jiki, ƙara 50ul CY5 ruwa don elution, gauraya da kyau, sanya shi a kan ma'aunin maganadisu kuma bar shi ya tsaya don 30s, sa'an nan kuma canja wurin ruwa zuwa sabon tube na centrifuge.
⑨.Auna OD
3. Matakin cire miya
① Centrifuge a 12000rpm na 1min tare da miya da cakudewar adanawa
② Rike ruwan sama da kuma cire abin da ya fi karfin
③.Sai a zuba 0.6ml na ruwa CY1 da 10ul na proteinase k a ciki, sai a gauraya da kyau, sai a sanya shi a cikin injin iska a digiri 65 a ma'aunin celcius kuma a sanya shi na tsawon minti 30.
④ Centrifuge a 12000rpm na 1min, cire duk abin da ake buƙata zuwa sabon bututun centrifuge, ƙara 10ul Magnetic beads da 0.25ml CY2, gauraya sosai don 12min, sanya shi a kan ma'aunin maganadisu kuma bar shi ya tsaya tsawon 30s don tsotse ruwan.
⑤A zuba 0.6ml na ruwa CY3, a gauraya da kyau na tsawon mintuna 3, sai a dora a kan ma'aunin maganadisu sai a bar shi ya tsaya tsawon 30s don tsotse ruwan.
⑥.Ƙara 0.6ml na ruwa CY4, gauraya na minti 3, sanya shi a kan ma'aunin maganadisu kuma bar shi ya tsaya don 30s don tsotse ruwan.
⑦.Maimaita mataki ⑥
⑧.A bushe a dakin da zafin jiki na minti 10-20, ƙara 50ul na ruwa na CY5 don elution, haɗuwa da kyau, sanya shi a kan ma'auni na Magnetic kuma bar shi ya tsaya don 30s, sa'an nan kuma canja wurin ruwa zuwa sabon tube na centrifuge.
⑨.Auna OD
Lura: Idan kana buƙatar cire RNA, zaka iya shirya RNaseA 10mg/ml: ƙarfi (10mM sodium acetate: pH5.0), tafasa shi don 15min, daidaita pH 7.5 tare da Tris-Hcl, kuma adana a -20 digiri Celsius.
Yanayin ajiya da lokacin inganci
1. Ya kamata a yi amfani da samfurin a cikin yanayi mai tsabta da tsabta, yana guje wa gurbatawa, kuma yana da zafin jiki mai dacewa.
2. Ajiye a dakin da zafin jiki.Ana iya adana furotin K da beads na maganadisu a 2-8 ° C na dogon lokaci.
3. Rayuwa rayuwar samfur: 12 watanni
Matakan kariya:
1. Ana amfani da wannan samfurin kawai don ganewar asali na in vitro.
2. Yanayin ajiya da matakan cirewa yakamata su bi umarnin da ke cikin littafin.
3. Idan ka ga cewa adadin ya yi ƙanƙara a lokacin cirewa, zaka iya ƙara girman samfurin daidai ko ƙara yawan adadin abubuwan da aka cire.
4. Dole ne DNA ɗin da aka fitar ya zama sabo kuma an gwada shi cikin lokaci.
Lura: Ana amfani da wannan hanyar sufuri don bincikar in vitro, kuma ba za a iya amfani da ita don amfani na ciki ko na waje a cikin mutane ko dabbobi ba.Idan aka haɗiye, yana iya haifar da munanan al'amura;yana da haushi ga idanu da fata.Idan bazata fantsama cikin idanu ba, a wanke da ruwa.Ya kamata a ba da iska yayin amfani.
Gabatarwar masana'anta
Huachenyang (Shenzhen) Fasaha Co., Ltd. ya ƙware a cikin samar da swabs, maƙogwaro, swabs na baki, hanci swabs, mahaifa swabs, soso swabs, ƙwayoyin cuta Samfur tubes, cutar adana mafita.Yana da wasu ƙarfi a cikin masana'antar.mai kyau
Muna da fiye da shekaru 12+ na Ƙwarewar Masana'antu a cikin Kayayyakin Kiwon Lafiya
HCY yana ɗaukar ingancin samfurin a matsayin mahimmancin ci gaban kasuwanci, yana manne wa shugaban "kayayyakin farko, sabis na aji na farko" ta kowane yanayi, yana bin ruhin kasuwancin "neman gaskiya, ƙirƙira, haɗin kai da inganci" .HCY tana tsara duk tsarin samarwa da tallace-tallace daidai da tsarin gudanarwa na ISO9001 da ISO13485, tare da ingantaccen aiki da ingantaccen inganci.
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